Pairwise was recently published in the paper by Clare et. al, "Development of KASP Fingerprinting Panel for Clonal Identification in Red Raspberry (Rubus ideas L.)" Pairwise's own, Cherie Ochsenfeld, was a key contributor in this collaborative work with the USDA.
To learn more about this innovative initiative to identify diversity in red raspberries, read the full paper on Plant Breeding.
Abstract: Red raspberry is an economically important horticultural crop that is known for its fruit's sweet flavour and nutritional value. A reliable and economic genotyping platform is needed to facilitate clonal/variety identification. Previous attempts for clonal identification utilized morphological traits or low-throughput, difficult to score dinucleotide-containing simple sequence repeat molecular markers. Single nucleotide polymorphisms (SNPs), despite having lower allelic diversity, are numerous across the genome and more easily converted to high-throughput assays restoring differential power. In this study, we use the kompetitive allele-specific PCR (KASP™) chemistry, an affordable and high-throughput platform, to develop a panel of SNPs to distinguish a diverse collection of red raspberry accessions for clonal identification. The panel consists of 48 KASP assays that show high concordance with whole genome sequencing, allelic balance, and recovery rate and a minimal set of 24 assays that distinguished the same accessions differentiated by the larger panel.